HPTLC method for analysis of withaferin-A in Ashwagandha<i>(Withania somnifera)</i>

Abstract

A densitometric high-performance thin layer chromatographic (HPTLC) method has been established for analysis of withaferin-A in Withania somnifera . The analyte was extracted with methanol. Withaferin-A standard and sample was spotted by use of a sample applicator. The plates were developed with toluene-ethyl acetate-formic acid 5:5:1 as mobile phase. Quantitative evaluation was performed by measuring the absorbance of the analyte zones at 200 nm in reflectance mode. The method was shown to have the selectivity, accuracy, precision, and high sample throughout useful for routine analysis of the preparation in industrial quality control and regulatory laboratories.