Voltammetric Behavior of Methotrexate Using Mercury Meniscus Modified Silver Solid Amalgam Electrode

Abstract

Abstract Voltammetric behavior of methotrexate (MTX), an important chemotherapeutic drug, has been investigated using electrochemical methods: cyclic voltammetry, direct current voltammetry and differential pulse voltammetry on a mercury meniscus modified silver solid amalgam electrode (m‐AgSAE). The obtained results have been compared with those obtained using a hanging mercury drop electrode (HMDE). The optimal conditions for its determination have been found in a Britton‐Robinson buffer of pH 5 with the limit of detection equal to 1.8 nM. RSD at this concentration level amounted to less than 4 %. The proposed procedure of determination was verified on a real drug containing MTX.