Development of sequence‐tagged microsatellites for the barley net blotch pathogen, <i>Pyrenophora teres</i>

Abstract

Abstract A modified sequenced‐tagged microsatellite (STM) profiling procedure was used to develop 80 STMs for the barley net blotch pathogen, Pyrenophora teres . Of these, 60 STMs amplified 67 loci in one or both of the spot ( P. teres f. maculata ) and net ( P. teres f. teres ) forms of the pathogen. When screened on six field‐sampled isolates of each pathogen form, 25 STMs revealed 26 polymorphic loci, with an average of 3.2 ± 1.0 alleles and mean gene diversity of 0.59 ± 0.12.