Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography

Abstract

Rat plasma, containing 125I-labeled triglyceride-rich lipoprotein, was mixed following lipid extraction with 10% SDS buffer and analyzed by gel filtration chromatography on columns using an elution buffer containing 1% SDS. Labeled apoproteins were separated into apo B, apo E, and apo C radioactivity peaks. Labeled peptides, tyrosine, and iodide were also resolved by this method. Isolated lipoprotein fractions were separated into the same components. The method offers the advantages of quantitative radioactivity recovery, large sample volume, and resolution of two apo B proteins.