Annals of Neurology · 2006 · 38 citations · 7 references
Life SciencesBiochemistryNeurologyClinical BiochemistryNeurodegenerationProteomicsCerebrospinal Fluid BiomarkersMultiple SclerosisCerebrospinal Fluid AnalysisCerebrospinal Fluid MarkersCerebrospinal Fluid Neurodegeneration MarkersCerebrospinal Fluid Protein StudiesCleavage FormationCystatin CDiagnostic Marker DiscoveryMultiple Sclerosis Pathology
OBJECTIVE: A recent study using surface-enhanced laser desorption/ionization time-of-flight analysis of cerebrospinal fluid identified a 12.5 kDa truncated isoform of cystatin C (CysC) as a specific biomarker for multiple sclerosis (MS). METHODS: Surface-enhanced laser desorption/ionization time-of-flight analysis of cerebrospinal fluid samples from 43 MS patients and 46 healthy control subjects. RESULTS: Full-length CysC (13.4 kDa) concentration was similar in MS and control samples. The 12.5 kDa CysC protein was produced from full-length CysC by N-terminal cleavage during storage at -20 degrees C. INTERPRETATION: The 12.5 kDa CysC isoform is a storage-related artifact and is not useful as a diagnostic marker for MS.
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Rosamonde E. Banks, Anthea J. Stanley, David A. Cairns et al. · Clinical Chemistry · 2005 · 240 citations · Full text