Type II Isopentenyl Diphosphate Isomerase from<i>Synechocystis</i>sp. Strain PCC 6803

Abstract

ABSTRACT Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His 6 -tagged protein was produced in Escherichia coli and purified by Ni 2+ chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg 2+ for activity; K m IPP was 52 μM, and k cat IPP was 0.23 s −1 .