Surface Modification via Plasma Polymerization of Allylamine for Antibody Immobilization

Abstract

Abstract Continuous wave and pulsed plasma polymerization coatings of allylamine were investigated for antibody immobilization as a function of plasma power, monomer pressure and treatment time, and duty cycle. Conditions were optimized by evaluating the surface amine density of plasma polymer coated samples before and after aging in dry ethanol for 3 hrs. In addition, plasma polymer coatings were characterized by contact angle analyzer, α‐step, and FT‐IR/ATR. The continuous wave plasma polymerization provided amine density of 4.8 molecules/nm 2 at the optimum condition of 20 W, 1 min, and 60 mTorr, while the pulsed plasma polymerization coating resulted in further increased amine density to 5.2 molecules/nm 2 at the duty cycle of 60/100.