Diversity Arrays: a solid state technology for sequence information independent genotyping

TLDR

The study demonstrates the application of Diversity Array Technology (DArT) to DNA polymorphism analysis, using the rice genome as a model to showcase its high‑throughput potential. DArT assays the presence or quantity of specific DNA fragments from a genomic representation on solid‑state arrays, employing either dual‑representation contrast or contrast against a universal reference fragment. The resulting Diversity Panels enable rapid, economical genetic fingerprinting of any organism within the source gene pool and support highly parallel genotyping of any genome or complex mixture.

Abstract

Here we present the successful application of the microarray technology platform to the analysis of DNA polymorphisms. Using the rice genome as a model, we demonstrate the potential of a high-throughput genome analysis method called Diversity Array Technology, DArT‘. In the format presented here the technology is assaying for the presence (or amount) of a specific DNA fragment in a representation derived from the total genomic DNA of an organism or a population of organisms. Two different approaches are presented: the first involves contrasting two representations on a single array while the second involves contrasting a representation with a reference DNA fragment common to all elements of the array. The Diversity Panels created using this method allow genetic fingerprinting of any organism or group of organisms belonging to the gene pool from which the panel was developed. Diversity Arrays enable rapid and economical application of a highly parallel, solid-state genotyping technology to any genome or complex genomic mixtures.