Maternal Exposure to Triclosan Impairs Thyroid Homeostasis and Female Pubertal Development in Wistar Rat Offspring

Abstract

Although the effects of triclosan have been examined in male reproductive functions, it is unknown whether this potent antibacterial agent affects pregnancy and female pubertal development. Effects of maternal exposure to triclosan on thyroid homeostasis (TH) and reproductive-tract development in female Wistar rats were thus studied. Dams were exposed daily to triclosan (0, 1, 10, or 50 mg/kg/d) from 8 d before mating to lactation day 21. Offspring were also exposed after weaning. In vivo triclosan estrogenic activity was screened by uterotrophic assay and vaginal opening (VO), with first estrus and uterus and ovarian weight determined in offspring. Dam blood samples were taken during pregnancy and lactation to examine the effect of triclosan on TH. No apparent external signs of toxicity or differences in mean numbers of implantation sites were observed in treated rats. Triclosan treatment decreased total serum T(4) and T(3) in pregnant rats and also lowered sex ratio, lowered pup body weights on postnatal day (PND) 20, and delayed VO in offspring. In addition, the highest dose of triclosan significantly reduced the live birth index (percentage) and 6-d survival index. Data indicate that triclosan impairs thyroid homeostasis and reproductive toxicity in adult rats and produces fetal toxicity in offspring exposed in utero, during lactation, and after weaning.