Molecular Plant-Microbe Interactions · 2001 · 125 citations · 15 references
AgriculturePlant BiologyPlant SciencesGene ExpressionHost-pathogen InteractionsPlant Molecular BiologyBacterial Pathogenesis In PlantsPlant Defense ResponsesPlant Disease ResistancePlant Immune ResponsesPlant PathogenesisPlant-pathogen InteractionsConstitutive Gene ExpressionDefense-related Gene ExpressionPathogen Resistance InductionPathogen-induced Gene ExpressionPathogenesis-related Gene ExpressionPlant Defense Gene ExpressionTranscriptional ActivationTranscriptional Activation Domains
The tomato Pti5 gene encodes a pathogen-inducible ethylene response element-binding protein-like transcription factor that interacts with the disease resistance gene product Pto. Overexpression of Pti5 or Pti5-VP16, a translational fusion with a constitutive transcriptional activation domain, in tomato enhanced resistance to Pseudomonas syringae pv. tomato. Constitutive expression of Pti5 or Pti5-VP16 did not affect the basal level of pathogenesis-related gene expression, but it accelerated pathogen-induced expression of GluB and Catalase. The results demonstrate a positive role of Pti5 in defense gene regulation and disease resistance and suggest that a pathogen-activated posttranscriptional regulatory step is necessary for the pathogen induction of the defense gene expression.
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