Serotype-Specific Detection of Coxsackievirus A16 in Clinical Specimens by Reverse Transcription-Nested PCR

Abstract

We describe the development of a coxsackievirus A16 (CVA16) serotype-specific PCR which correctly differentiated between CVA16 and other enterovirus serotypes of both laboratory isolates and clinical specimens. The assay will be useful for monitoring CVA16 outbreaks and studying the disease association, epidemiology, and evolution of this common enterovirus serotype.