IEEE Transactions on Biomedical Engineering · 2004 · 127 citations · 37 references
EngineeringNeurobiologyNeuroscienceElectrophysiologyNeural CircuitryNeural EngineeringHippocampal CircuitsMicroelectrode ArraysNeural InterfacesNeural Recording TechnologiesNeural Signal AcquisitionNeuronal Cell StructureBursting ActivityElectrophysiological RecordingsExtracellular Neural RecordingExtracellular RecordingIn Vitro Neural RecordingMicrocontact PrintingMicroelectrode Array RecordingNeural Recording
Neuronal cell networks have been reconstructed on planar microelectrode arrays (MEAs) from dissociated hippocampal pyramidal neurons. Microcontact printing (microCP) and a photoresist-liftoff method were used to selectively localize poly-L-lysine (PLL) on the surface of MEAs. Haptotaxis led to the organization of the neurons into networks localized adjacent to microelectrodes. Various grids of PLL with 2-25-microm-wide lines spaced by 50-200 microm with 15-25-microm nodes at intersection points were used to guide cell body attachment and neurite outgrowth. Bursting activity with spike amplitude attenuation was observed, and multichannel recordings detected instances of coincident firing activity. Finally, we present here an extracellular recording from a approximately 2 microm bundle of guided neurites.
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