Development and Performance Evaluation of a Tandem Mass Spectrometry Assay for 4 Adrenal Steroids

Abstract

The detection limit and selectivity of this method and its small sample volume requirement allow analysis of endogenous concentrations of adrenal steroids in serum or plasma from children and adults. The method thus has an important potential role in the evaluation of the status of 4 of the enzymes involved in adrenal steroid biosynthesis.