INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 2005 · 279 citations · 38 references
Life SciencesMicrobiologyMolecular BiologyBacteriologyPhylogeneticsSequence Analysis16S Ribosomal Ribonucleic Acid AnalysisBacterial IdentificationMicrobial PhylogeneticsMicrobial PhylogenyMolecular PhylogenyPhylogenomic Analysis16S Ribosomal Rna Gene Analysis16S Rrna Gene PhylogenyDna Sequencing For IdentificationMultigene FamiliesMycobacterial DiseasePhylogenetic Character AnalysisPhylogenetic Tree InferenceRibosomal Dna Sequencing
Advances in DNA sequencing and the increasing number of sequences available in databases have greatly enhanced the bacterial identification process. Several species within the genus Mycobacterium cause serious human and animal diseases. In order to assess their relative positions in the evolutionary process, four gene fragments, from the 16S rRNA (564 bp), hsp65 (420 bp), rpoB (396 bp) and sod (408 bp) genes, were sequenced from 97 strains, including all available type strains of the genus Mycobacterium. The results demonstrate that, in this case, the concatenation of different genes allows significant increases in the power of discrimination and the robustness of the phylogenetic tree. The sequential and/or combined use of sequences of several genes makes it possible to refine the phylogenetic approach and provides a molecular basis for accurate species identification.
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Julie Thompson, Desmond G. Higgins, Toby J. Gibson · Nucleic Acids Research · 1994 · 64.9K citations · Full text