Specific Protein Docking to Chelator Lipid Monolayers Monitored by FT‐IR Spectroscopy at the Air–Water Interface

Abstract

A novel class of chelator lipids combines the fundamental properties of self-assembly with protein engineering techniques, leading to well-organized biofunctionalized interfaces and two-dimensional protein arrays. Reflection FT-IR spectroscopy at the air - water interface was used to monitor the formation of a metal-sensitive lipid film as well as the reversible and specific docking (represented on the right) of a fusion protein (B) to this chelator lipid layer through a histidine tag (A).