Calcium Alginate: A Support Material for Immobilization of Proteases from Newly Isolated Strain of Bacillus subtilis KIBGE-HAS

Abstract

Abstract: Partially purified neutral protease was entrapped in calcium alginate beads and characterized using casein as a substrate. Temperature and pH maxima of the enzyme showed no changes before and after immobilization and remained stable at 50°C and 7.5 respectively. However substrate concentration for maximum enzyme activity of immobilized enzyme was shifted from 0.45 % to 0.8%. Reaction time for immobilized enzyme assay was also increased by 5 minutes with reference to soluble enzyme i.e., from 15 to 20 minutes. Enzyme activity was decreased when the concentration of alginate was increased above 2%. Immobilized enzyme retained its activity for longer time and can be reused upto three times. The storage stability of entrapped protease at 4°C was found upto 10 days, while at 30°C the enzyme lost its activity within three days. Key words: Alginate beads Bacillus subtilis Immobilization Protease GenBank: EU819145