Site‐Specific Engineering of Chemical Functionalities on the Surface of Live Hepatitis D Virus

Abstract

Precise virus labeling: The genetic code expansion strategy was coupled with an engineered virus assembly process in human hepatocytes to produce intact human hepatitis D virus (HDV) bearing a genetically encoded unnatural amino acid. The resultant HDV virions, with one type of five different pyrrolysine analogues introduced site-specifically into virus surface proteins, exhibited near wild-type viability and infectivity. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.