Plant tissue culture study on two different races of purslane (Portulaca oleracea L.)

Abstract

  This study was performed on two races of purslane; agronomic purslane and wild one. All the explants were cultured on MS basal medium supplement with 30 g/l sucrose, 8 g/l agar and different plant growth regulator. Petioles, shoot tips and leaves of wild purslane and also leaves of agronomic purslane were cultured in different concentrations and combinations of IBA and BAP (Both in the same four levels, 0, 1, 5 and 10 µM; collectively 16 treatments). On the other hand, nodal segments and shoot tips of agronomic purslane were cultured in different concentrations (4.44, 8.88 and 13.32 µM) of BAP or kinetin. The regenerated were rooted in the media containing three levels of IBA or IBA (0.0, 2.5 and 5 µM). Results showed that the treatments containing10 µM IBA in combination with 10 or 5 µM BAP are suitable for callus induction from leaves of wild purslane. Direct shoot regeneration from shoot tips or petiole explants of wild purslane was observed only in 10 µM IBA alone. Also, BAP at level 8.88 µM was found to be the best treatment to shoot regeneration from nodal segments of agronomic purslane and IBA at level 2.5 µM was found to be the best treatment for rooting of regenerated shoots in both races of purslane.     Key words: Portulaca oleracea, microproagation, callus induction, BAP, NAA, IBA.