UPS 2.0: unique probe selector for probe design and oligonucleotide microarrays at the pangenomic/ genomic level

Abstract

The UPS 2.0 evaluates probe-to-target hybridization under a user-defined condition to ensure high-performance hybridization with minimal chance of non-specific binding at the pangenomic and genomic levels. The UPS algorithm mimics the target/non-target mixture in an experiment and is very useful in developing diagnostic kits and microarrays. The UPS 2.0 website has had more than 1,300 visits and 360,000 sequences performed the probe designing task in the last 30 months. It is freely accessible at http://array.iis.sinica.edu.tw/ups/. Screen cast: http://array.iis.sinica.edu.tw/ups/demo/demo.htm.