Covalent Enzyme Immobilization onto Photopolymerized Highly Porous Monoliths

Abstract

Enzymes covalently immobilized on highly porous materials are demonstrated to have very high biocatalytic activity and good recyclability, exemplified by Candida antarctica Lipase B (CAL-B). Polymerized high internal phase emulsions (PolyHIPEs, see figure) are developed for covalent grafting of proteins (enzymes) via the reaction of the protein surface lysine residues with active ester moieties in the monolithic material. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2089/2006/c0293_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.