Role of Activated Macrophages in Resistance of Mice to Infection with Trypanosoma cruzi

Abstract

The role of the activated macrophage in resistance of mice to infection with Trypanosoma cruzi was investigated with use of mice that had not been infected with T. cruzi but whose macrophages were activated by Toxoplasma gondii or Besnoitia jellisoni. Mice with activated macrophages were significantly more resistant to intra-peritoneal challenge with the Tulahuen strain of T. cruzi than were controls. The role of serum factors in resistance to T. cruzi was excluded by the demonstration of no difference between survival of T. cruzi in serum from mice with activated macrophages and in that from controls. As the only modality of resistance to T. cruzi in this in vivo model appeared to be the activated macrophage, this cell was investigated in vitro. Activated macrophages were shown to be able to inhibit completely multiplication of T. cruzi, whereas significant multiplication of the parasite occurred in control macrophages. Both in vivo and in vitro data suggest that the activated macrophage may play a major role in resistance to infection with T. cruzi.