Sensing Phosphatase Activity by Using Gold Nanoparticles

Abstract

To change your colors: A colorimetric enzyme assay based on peptide-induced aggregation of gold nanoparticles (Au-NPs) has been developed to sense phosphatase activity. Once the phosphate group is eliminated by alkaline phosphatase, peptide-induced Au-NP aggregation is triggered, resulting in a significant change in absorbance at 650 nm. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z603735_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.