Nucleic Acids Research · 1988 · 116 citations · 39 references
Life SciencesGeneticsMolecular BiologyGene ExpressionProtein-protein InteractionsTranscriptional RegulationGene Regulatory ElementsNucleic Acid-protein InteractionsPromoter RecognitionPromoter RegulationProtein-nucleic Acid InteractionsTranscription FactorsDna InteractionsEquilibriumGene Promoter BindingPromotersProtein-dna Binding Site PredictionTranscription Factor Binding At PromotersTranscription Factor Binding SitesTranscription Factor Binding Specificity
Nuclear factor I (NFI) was purified to homogeneity from porcine liver by DNA-affinity chromatography and displays a single band with a molecular weight of 36 kDa in SDS-polyacrylamide gels. The purified protein was used to determine absolute equilibrium binding constants by gel retardation techniques for a variety of DNA fragments with genuine or mutated NFI binding sites and a number of DNA fragments derived from various eukaryotic promoters carrying the CCAAT-box as a half-site for NFI binding. We present a model which allows prediction of the functional significance of mutated NFI binding-sites from sequence data. The data suggest that the single molecular species of NFI from porcine liver may not be able to recognize and activate the -CCAAT- promoter element in vivo without additional interactions, e.g. with other proteins.
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Ulrich K. Laemmli · Nature · 1970 · 251.6K citations
Haider Raza, Annie John, Abderrahim Nemmar · Cellular Physiology and Biochemistry · 2013 · 47.3K citations · Full text