Genes & Development · 2007 · 202 citations · 33 references
Life SciencesGeneticsMolecular BiologyChromatin BiologyGene RegulationTranscriptional RegulationChromatinNotch SignalingPromoter RegulationTranscription FactorsTranscriptional ControlTranscriptional Regulation In EukaryotesChromatin-associated Protein InteractionsEnhancersNotch Signaling ActivationNotch Target GenesRna Polymerase Ii FunctionRna Polymerase Ii RecruitmentSelective Binding Sites
The CSL [CBF1/Su(H)/Lag2] proteins [Su(H) in Drosophila] are implicated in repression and activation of Notch target loci. Prevailing models imply a static association of these DNA-binding transcription factors with their target enhancers. Our analysis of Su(H) binding and chromatin-associated features at 11 E(spl) Notch target genes before and after Notch revealed large differences in Su(H) occupancy at target loci that correlated with the presence of polymerase II and other marks of transcriptional activity. Unexpectedly, Su(H) occupancy was significantly and transiently increased following Notch activation, suggesting a more dynamic interaction with targets than hitherto proposed.
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Histone Variant H2A.Z Marks the 5′ Ends of Both Active and Inactive Genes in Euchromatin
Ryan Raisner, Paul D. Hartley, Marc D. Meneghini et al. · Cell · 2005 · 680 citations · Full text