American Journal of Medical Genetics · 1991 · 70 citations · 19 references
Life SciencesGeneticsMolecular GeneticsGenetic CounselingHuman GeneticsPrenatal DiagnosisCongenital AnomaliesCongenital DisordersCongenital MalformationsFetal AnomaliesGenetic DisordersAlpha Thalassemia Prenatal DiagnosisCompound Heterozygosity AnalysisDna Hybridization AssaysHomozygous DeletionMolecular CharacterizationMolecular Diagnosis Of HemoglobinopathiesPerinatal Death RatePrenatal Molecular Diagnosis
alpha-Thalassemia hydrops fetalis is a common disorder in Taiwan. The condition causes perinatal death and many maternal obstetrical complications. In order to determine the molecular defects of this condition in Chinese, 87 unrelated families with this disorder were collected in the past 4 years. The molecular defects were studied by Southern blotting and DNA hybridization with phi zeta 1-globin gene and LO (a 0.4 kb BamHI/EcoRI fragment in the 5' flanking region of the zeta 2-globin gene) probes. Eighty-one (93.1%) fetuses had homozygous Southeast Asian deletion (- -SEA/- -SEA). Five (5.7%) fetuses were compound heterozygotes for the Southeast Asian deletion and Thailand deletion (- -SEA/- -THAI). The remaining fetus was a compound heterozygote for the Southeast Asian deletion and an uncharacterized nondeletional defect (- -SEA/(alpha alpha)Th). The molecular defects of alpha-thalassemia hydrops fetalis in Chinese are heterogeneous. This fact has important implications for genetic counseling and prenatal diagnosis.
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