Analytical Letters · 1991 · 38 citations · 13 references
EngineeringBiochemistryBiotechnologyBiosensorsElectroanalytical MethodsEnzymologyElectrochemical BiosensorsElectrochemical DetectionElectrochemical SensorsEnzymatic BiosensorsEnzyme-based BiosensorsMarine BiosensorsElectrochemical Enzyme SensorsEnzymatic SensorsEnzyme ActivityOrganophosphate BiosensorsPotentiometric Enzymatic BiosensorsPotentiometric Enzyme BiosensorsPotentiometric MeasurementPotentiometric Sensors
Abstract Biotinylated acetylcholinesterase (AChE) from Eel was immobilized to a biotinylated cellulose nitrate membrane via a streptavidin bridge. Using a light addressable potentiometric sensor (LAPS), enzyme activity was determined as a function of substrate concentration (i.e. acetylcholine) and amount of enzyme immobilized to the membrane. This biosensor was also evaluated for its potential to detect organophosphate (OP) anticholinesterases (antiChEs). Key Words: LAPSAnticholinesteraseBiosensorAcetylcholinesteraseLAPSAnticholinesteraseBiosensorAcetylcholinesterase
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