COMPARISON OF THE CONJUGATED METABOLITES OF INTRAVENOUSLY AND INTRADUODENALLY ADMINISTERED OESTRIOL

Abstract

ABSTRACT The metabolism of oestriol-16- 14 C( 14 C-OE 3 **) and oestriol-15- 3 H( 3 H-OE 3 ) was studied in a 28 year-old man with normal liver function, who had undergone cholecystectomy and whose bile was being drained via a Ttube. 14 C-OE 3 was injected into the duodenum through the T-tube and shortly later, 3 H-OE 3 was injected into the antecubital vein. Bile and urine were collected continuously at intervals for 30 and 43 hours respectively. Each fraction was assayed. The excretion of 3 H was more rapid than that of 14 C in both fluids. Twenty per cent of the injected 3 H and 17 per cent of the 14 C were found in the bile, while 55 per cent of the 3 H and 76 per cent of the 14 C were excreted in the urine. Isolation and identification of the conjugated metabolites were carried out by the use of column chromatography on alumina and celite, paper