PHOTOCHEMICAL LABELING OF YEAST ALCOHOL DEHYDROGENASE WITH AN AZIDE ANALOG OF NAD +

Abstract

Abstract— 3‐Azidopyridine adenine dinucleotide, an azide analog of NAD + , has been synthesized as a potentially general photochemical labeling reagent for the active sites of NAD‐dependent dehydrogenases. The analog is a competitive inhibitor of NAD reduction by yeast alcohol dehydrogenase (YADH). Upon photolysis of the 3 H‐analog in the presence of YADH, 7% of the label becomes covalently bound. The results are discussed in terms of desired properties of a photochemical labeling reagent.