Development of an ethidium monoazide–enhanced internally controlled universal 16S rDNA real‐time polymerase chain reaction assay for detection of bacterial contamination in platelet concentrates

Abstract

Cross-linking of EMA to DNA via photoactivation solved the previously intractable problem of reagent contamination and permitted the development of a high-sensitivity universal bacterial detection system. Trials are ongoing to assess the suitability of the system for high-throughput screening of PLT concentrates.