Isotope‐labeled differential profiling of metabolites using <i>N</i> ‐benzoyloxysuccinimide derivatization coupled to liquid chromatography/high‐resolution tandem mass spectrometry

Abstract

This simple derivatization reaction can be applied to the relative quantitation of amine-, thiol- and phenol-containing compounds, with improved sensitivity and chromatographic peak shapes due to the increased hydrophobicity of polar metabolites not readily amenable to reversed-phase LC/MS analysis.