The Occurrence of an Inhibitor of Ca2+-Dependent Neutral Protease in Rat Liver1

Abstract

The occurrence of a novel and specific inhibitor of Ca2+-dependent neutral protease in rat liver has been demonstrated. The partially purified product is a heat-stable and acid-stable protein of an approximate molecular weight of 3x10(5), and readily inactivated by tryptic digestion. The inhibition of Ca2+-dependent protease is not a result of the binding of Ca2+ by the inhibitor.