Design and Synthesis of a Homogeneous Erythropoietin Analogue with Two Human Complex‐Type Sialyloligosaccharides: Combined Use of Chemical and Bacterial Protein Expression Methods

Abstract

Highly expressive: Cell proliferation was observed with concentrations of an erythropoietin (EPO) analogue above 50 pg mL−1. The EPO analogue has two human complex-type sialyloligosaccharides (see picture) and was formed by the combined use of chemical synthesis and protein expression in E. coli. Both the 24- and 30-positions are glycosylated, but the two sialyloligosaccharides do not interfere with binding of the EPO analogue to a receptor. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.