Production and characterization of monoclonal antibodies to antigens from second stage juveniles of the potato cyst nematode, Globodera rostochiensis

Abstract

Summary - FoHowing immunization of mice with different antigens from Globodera rosLOchiensis, monoclonal antibodies (MAbs) were screened by fluorescence microscopy for reaction with specific srructures in second stage juveniles (J2) of G. roslOchiensis. MAbs were obtained which bound to the subvenrraJ oesophageal glands, the body-wall muscle filaments, the genital primordium, the intestinal lumen, ceU nuclei, and the cuticle surface. The MAbs 10 the subvenrral glands also bound 10 the subvenrraJ glands inJ2 of G. paUida and G. tabacum, but not in J2 of Heterodera glycines, H. SChaChlu~ kleloidogyne hapla or M. incognita. Three subvenrral gland l'viAbs reacted with a water soluble epitope in native protein exrracts from J2 of G. rosLOchiensis. The MAbs to the body-waU muscle filaments also bound 10 the body-wall musculature in J2 of G. paUida, H. schachtu~ M. hapla, and M. incognita. On Western blots of J2 of G. rOSlOchiensis these MAbs reacted with t'Wo proteins of 39 kDa and> 106 kDa respectively.