<b>INTRALOBULAR AND INTRACELLULAR LOCALIZATION OF </b><b>ALCOHOL DEHYDROGENASE (ADH) ISOZYMES IN MOUSE LIVER: BASIC ADH (CLASS I) AND ACIDIC ADH (CLASS III) </b>

Abstract

Localization of alcohol dehydrogenase isozymes [basic alcohol dehydrogenase (ADH): Class I and acidic ADH: Class III] was studied at both lobular and cellular levels in mouse liver by using immunohistological methods. The immunofluorescent microscopy showed that the basic ADH is distributed more predominantly in the perivenous zone than in the periportal zone, although the enzyme was detected in every parenchymal cell within the whole hepatic lobule. By immunoelectron microscopy, the enzyme was shown to distribute within the cytoplasmic matrix of hepatocytes, except for glycogen areas. On the other hand, acidic ADH was found to localize mainly on the periphery of the parenchymal cell, showing no dis-