Publication | Open Access
Complete Assignment of Heteronuclear Protein Resonances by Protonless NMR Spectroscopy
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Citations
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References
2005
Year
Cβ NucleiBiochemistryMagnetic Resonance SpectroscopyNatural SciencesBiomolecular AnalysisResonanceMagnetic ResonanceMolecular BiologyNucleic Acid BiochemistryHigh-resolution Nmr SpectroscopyProtonless Nmr SpectroscopySide-chain NucleiProtein NmrAnalytical UltracentrifugationSolution Nmr SpectroscopyNuclear Magnetic Resonance SpectroscopyMedicineBiophysics
The detection of 13C is now amenable to high-resolution NMR spectroscopy of proteins. A protocol for 13C homonuclear decoupling in multidimensional experiments correlates carbonyl, Cα, and Cβ nuclei (see picture). These techniques also allow the detection of side-chain nuclei in exclusively heteronuclear experiments, and thus the complete assignment of proteins.
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