In Vitro and In Vivo Uncaging and Bioluminescence Imaging by Using Photocaged Upconversion Nanoparticles

Abstract

Trading up: A bioimaging system that is based on caged D-luciferin/upconversion nanoparticle conjugate has been developed. The nanoparticles upconvert near-infrared light into UV light, which triggers the photorelease of D-luciferin (see scheme) and leads to enhanced fluorescence and bioluminescence signals in vitro and in vivo. The use of near-infrared light enables deep penetration into tissue in vivo with minimum cellular damage. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.