Single Gold Nanoparticles as Real‐Time Optical Probes for the Detection of NADH‐Dependent Intracellular Metabolic Enzymatic Pathways

Abstract

A coat of many colors: Plasmon resonance Rayleigh scattering (PRRS) spectroscopy is applied to the in-situ monitoring of the NADH-dependent growth of Au@Cu core–shell nanoparticles (NPs). The scattering signal of the AuNPs is red shifted when they are coated with Cu (see scheme). In conjunction with dark-field microscopy, a colorimetric NADH-dependent biosensor was constructed and tested to screen an anticancer drug affecting cell NADH metabolism.