Cold Spring Harbor Symposia on Quantitative Biology · 1972 · 104 citations · 0 references
Life SciencesBiochemistryMolecular BiologyEnzyme MechanismEnzymologyMetabolismEnergy MetabolismEnzyme BiophysicsEnzyme CharacterizationEnzyme KineticsEnzyme StructureStructural EnzymologyEnzyme Structure-function AnalysisIsoenzyme ClassificationNad+-dependent EnzymesNad-dependent EnzymesSkeletal Muscle PhysiologyStructural ConstraintsSubunit AssemblySubunit Stoichiometry
In vertebrates, the NAD+-dependent enzyme lactate dehydrogenase (LDH) (EC 1.1.1.27) is a tetramer (mol wt 140,000) which catalyzes the interconversion of l(+) lactate and pyruvate. Two subunit types predominate, the M type in skeletal muscle and the H type in heart tissue. The subject of this study is the M4 isoenzyme from dogfish (Squalus acanthius).