Distribution and binding of novel photosensitizer 2-devinyl-2-(1-methoxyl-ethyl) chlorin f in human breast cancer cells MCF-7

Abstract

The interaction of novel Photosensitizer 2-devinyl-2-(1-methoxyl-ethyl) chlorin f (CPD4) with human breast cancer cells MCF-7 was studied by fluorescence spectrum and laser confocal scan microscopy (LCSM). The experimental results exhibit that fluorescence emission band of CPD4 in MCF-7 cells move to long wavelength about 5 nm compared with that in incubation solution, which suggest that CPD4 could enter into MCF-7 cells and bind them strongly by electrostatic or hydrogen binding interaction. The LCSM images of CPD4 in MCF-7 cells show that the binding of CPD4 in MCF-7 cells takes place in cellular membrane and mitochondria mainly. These results indicate that CPD4 can be of selective subcellular location, but not free ionic status in MCF-7 cells.CPD4 could be a kind of promising photosensitizer for Photodynamic therapy.