Negatively charged amino acid residues play an active role in orienting the Sec‐independent Pf3 coat protein in the Escherichia coli inner membrane

Rapid and efficient site-specific mutagenesis without phenotypic selection.

Thomas A. Kunkel · Proceedings of the National Academy of Sciences · 1985 · 7.3K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +15

[6] Use of T7 RNA polymerase to direct expression of cloned genes

F. William Studier, Alan H. Rosenberg, John J. Dunn et al. · Methods in enzymology on CD-ROM/Methods in enzymology · 1990 · 6.6K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +10

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

Stanley Tabor, C C Richardson · Proceedings of the National Academy of Sciences · 1985 · 3.2K citations · Full text

Life Sciences, Microbiology, Molecular Biology +14

Topogenic signals in integral membrane proteins

Gunnar von Heijne, Ylva Gavel · European Journal of Biochemistry · 1988 · 719 citations · Full text

Life Sciences, Biochemistry, Molecular Biology +17

Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues

Gunnar von Heijne · Nature · 1989 · 608 citations

Life Sciences, Biochemistry, Molecular Biology +12