Scandinavian Journal of Infectious Diseases · 2011 · 25 citations · 12 references
Veterinary MedicineInfectious DiseasesMicrobiologyDiagnostic MicrobiologyInfectious Disease EpidemiologyMedical MicrobiologyBacterial InfectionsDiagnostic BacteriologyInfectious Disease DetectionInfectious Disease Molecular TestingPathogen DetectionRespiratory Pathogen DiagnosticsBacterial Culture DiagnosisBacterial Culture DiagnosticsCough EtiologyDna ExtractionMolecular Respiratory Pathogen DetectionPathogen Dna DetectionPcr-based DetectionPolymerase Chain Reaction (Pcr) Assays
Pertussis, or whooping cough, is an acute respiratory disease mainly affecting infants and children and is caused by Bordetella pertussis and Bordetella parapertussis. The aim of this study was to investigate the share of Bordetella species from potential whooping cough cases during 2005-2009. Eight hundred and two samples from suspected pertussis cases were collected, mainly from 2 provinces of Pakistan. Bacterial culture, identification, DNA extraction and routinely used polymerase chain reaction (PCR) methods using IS1001, IS1002 and IS481 were used to identify the Bordetella species. The results were unexpected, because all of the isolates collected from the different cities were identified as B. parapertussis (7.4%); B. pertussis was not isolated from any sample. However, PCR results indicated the presence of a small percentage (0.6%) of B. pertussis among the total cases studied. This study suggests that vaccines to protect against both B. pertussis and B. parapertussis should be considered.
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Pertussis surveillance in French hospitals: results from a 10 year period
Isabelle Bonmarin, D Lévy-Brühl, S. Baron et al. · Eurosurveillance · 2007 · 78 citations · Full text
Anneke van der Zee, H. J. Groenendijk, Marc Peeters et al. · International Journal of Systematic Bacteriology · 1996 · 78 citations