Production of excreted human epidermal growth factor (hEGF) by an efficient recombinant Escherichia coli system

Molecular cloning: A laboratory manual

Prescott L. Deininger · Analytical Biochemistry · 1990 · 86.2K citations

Molecular Biotechnology, Natural Sciences, Genetics +8

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

Hermann Schägger, Gebhard von Jagow · Analytical Biochemistry · 1987 · 11.5K citations

Protein Chemistry, Biochemistry, Natural Sciences +8

Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA

Mark J. Zoller, Michael J. Smith · Nucleic Acids Research · 1982 · 761 citations · Full text

Genetics, Molecular Biology, Oligonucleotide-directed Mutagenesis +18

Isolation of the Insulin Receptor of Liver and Fat-Cell Membranes

Pedro Cuatrecasas · Proceedings of the National Academy of Sciences · 1972 · 396 citations

A PERSPECTIVE ON THE APPLICATION OF GENETIC ENGINEERING: STABILITY OF RECOMBINANT PLASMID

Tadayuki Imanaka, Shuichi Aiba · Annals of the New York Academy of Sciences · 1981 · 268 citations

Engineering, Genetics, Biotechnology +9