An Amphotericin B–Fluorescein Conjugate as a Powerful Probe for Biochemical Studies of the Membrane

Abstract

Not only skin deep: A fluorescein–amphotericin B conjugate with a new piperazine linker (see structure) was prepared and used as a probe of the biomembrane and the mechanism of action of amphotericin B both in vivo and in liposomal experiments. The amphotericin B analogue localizes at the membrane of yeast cells but is internalized by mammalian cells. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2004/z460489_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.