Regeneration of shoots from pea (<i>Pisum sativum</i>) hypocotyl explants

Abstract

A sequential indole‐3‐acetic acid (IAA)‐zeatin treatment was applied to Pisum sativum hypocotyl explants, resulting in shoot formation from 50% of the explants. Shoots were easily rooted and transplantable plants could be obtained in 3 months. The method has been applicable to the 5 cultivars tested. Histological examination of explants suggests the shoots to be of de novo origin, which would make the system suitable for transformation experiments.