Isolation and Inoculation of Endophytic Actinomycetes in Root Nodules of Elaeagnus Angustifolia

Abstract

8 strains of endophytic actinomycetes were successfully isolated from root nodules of Elaeagnus angustifolia by the methord of nodule-slicing respectively on three mediums S, FTW and DPM. And these strains were analyzed by 16S rDNA phylogenetic studies, the result showed that 5 of these strains belonged to Micromonospora, and the other 3 strains was Nonomuraea, Pseudonocardia and Planotetraspor, repectively.Inoculation of these isolated strains to the host plant, although no root nodules were obtained, the growth degree of the plant was obviously increased compared with the control.