Optimization of FTA technology for large scale plant DNA isolation for use in marker assisted selection

Abstract

TM technology uses a single format utilizing basic tools found in laboratory. In this study, FTA TM cards were used to collect over 3000 samples from a field located 130 km and used to screen progeny plants using PCR-marker-assisted selection. PCR product yields and quality are sufficient for reliable scoring, distinguishing heterozygous from homozygous plants using ABI 3730 sequencer. Results indicate that this method is faster, easier and in-expensive.