Antimicrobial Agents and Chemotherapy · 2007 · 496 citations · 17 references
Multiplex TechnologyPathogen DetectionPathologyDrug ResistanceMedical MicrobiologyInfection ControlMolecular DiagnosticsAntimicrobial ResistanceHealth SciencesMultiplex Pcr StrategySccmec TypingPathogen CharacterizationClinical MicrobiologySccmec Types IvAntimicrobial Resistance GeneAntimicrobial SusceptibilityMultiplex Pcr AssaysPathogenesisNucleic Acid AmplificationMicrobiologyMedicineDiagnostic Microbiology
Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.
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