American Journal of Tropical Medicine and Hygiene · 2002 · 225 citations · 20 references
Veterinary MedicineInfectious DiseasesMicrobiologyDiagnostic ParasitologyHelminthologyParasitologyFecal ExaminationHelminth DiagnosticsMolecular Diagnosis Of ParasitesParasitic Disease DiagnosisParasitic Infestation DetectionParasitic PathologyDetection Of Helminth ParasitesDna ExtractionKato-katz TechniqueMolecular Detection Of ParasitesMolecular Diagnosis Of Parasitic InfectionsParasitic DetectionParasitic HelminthsPolymerase Chain Reaction Specificity
A novel method for the detection of Schistosoma mansoni in human samples that is based on the amplification of a highly repeated DNA sequence has been developed. By use of simple DNA extraction techniques and a rapid 2-step polymerase chain reaction (PCR), it was possible to amplify S. mansoni DNA in human fecal and serum samples. The high sensitivity of the approach enabled the detection of the parasite DNA in fecal samples containing as few as 2.4 eggs per gram of feces, which makes it 10 times more sensitive than the Kato-Katz examination. A detection limit of I fg of Schistosoma sp. DNA was determined when pure DNA was used as PCR template. The amplification reaction showed to be specific giving no cross-reaction with DNA from other helminths. The PCR assay developed in this study may constitute a valuable alternative for the diagnosis of the Schistosoma sp. infection.
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A rapid one-tube genomic DNA extraction process for PCR and RAPD analyses
J. J. Steiner, C. J. Polemba, Robert G. Fjellstrom et al. · Nucleic Acids Research · 1995 · 164 citations