Combining Mass Spectrometry and Peptide Arrays to Profile the Specificities of Histone Deacetylases

Abstract

In profile: We describe the use of peptide arrays to probe the substrate specificities of several histone deacetylase (HDAC) enzymes. The combination with SAMDI-MS avoids the need for labels, which we demonstrate can perturb enzyme activity and lead to altered enzyme specificity. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.