Concepedia

Isolation and Functional Analysis of Human Neutrophils

Douglas B. Kuhns, Debra A. Long Priel, Jessica Chu, Kol A. Zarember

Current Protocols in Immunology · 2015 · 147 citations · 4 references

DOIFull text

Open access

Abstract

This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll-Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described.

References

4

Modulation of multiple neutrophil functions by preparative methods or trace concentrations of bacterial lipopolysaccharide.

Christopher Haslett, L A Guthrie, M M Kopaniak et al. · PubMed · 1985 · 781 citations · Full text

Toll-Like Receptor (TLR)2 and TLR4 in Human Peripheral Blood Granulocytes: A Critical Role for Monocytes in Leukocyte Lipopolysaccharide Responses

Ian Sabroe, Elizabeth C. Jones, Lynne R. Usher et al. · The Journal of Immunology · 2002 · 382 citations · Full text

Selective Roles for Toll-Like Receptor (TLR)2 and TLR4 in the Regulation of Neutrophil Activation and Life Span

Ian Sabroe, Lynne R. Prince, Elizabeth C. Jones et al. · The Journal of Immunology · 2003 · 342 citations · Full text

Monitoring Cell Growth

Warren Strober · Current Protocols in Immunology · 1997 · 82 citations

, , +15